Intersectional Genetic Reporters:


Using a novel intersectional reporter allele, Rosa-ai66 (MGI#3809505), developed by Hongkui Zeng at the Allen Institute for Brain Science, cells that co-express both Cre and Dre can be visualized by the dual-recombination events that allow for CAGGs-driven expression of a tdTomato reporter allele. Image from Devine et al., 2014, Elife.

Other intersectional strategies using different combinations of SSRs, such as Cre and Flp, have been well utilized by laboratories, such as that of Russell Ray here at BCM, to both amplify and attenuate cell function in an exquisitely precise fashion.

Cre diagram.png