FEATURED PUBLICATIONS

For a complete list of publications, please click here.

 
 

Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems

William Gillespie, Yuwen Zhang, Oscar E. Ruiz, Juan Cerda III, Joshua Ortiz-Guzman, Williamson D. Turner, Gabrielle Largoza, Michelle Sherman, Lili E. Mosser, Esther Fujimoto, Chi-Bin Chien, Kristen M. Kwan, Benjamin R. Arenkiel, W. Patrick Devine, Joshua D. Wythe

BioRxiv

DOI: https://doi.org/10.1101/2024.07.13.603267 / PMID: 39026881

 

MEK signaling represents a viable therapeutic vulnerability of KRAS-driven somatic brain arteriovenous malformations

Carlos Flores Suarez, Omar Ashraf Harb, Ariadna Robledo, Gabrielle Largoza, John J. Ahn, Emily K. Alley, Ting Wu, Surabi Veeraragavan, Samuel T. McClugage III, Ionela Iacobas, Jason E Fish, Peter T. Kan, Sean P. Marrelli, Joshua D Wythe

BiorXIV

DOI: https://doi.org/10.1101/2024.05.15.594335 / PMID: 38766159

 

Protocol for optical, aqueous clearing of murine tissues using EZ Clear

Taeyong Ahn, Gabrielle Largoza, Julia Younis, RE-JOIN Consortium, Mary E. Dickinson, Chih-Wei Hsu, Joshua D. Wythe

STAR Protocols

PMID: 38704833

 
 

GPU-Accelerated RSF Level Set Evolution for Large-Scale Microvascular Segmentation

Meher Niger, Helya Goharbavang, Taeyong Ahn, Emily K. Alley, Joshua D. Wythe, Guoning Chen, and David Mayerich

arXiv

PMID: 38654761

 

2023

 

The Role and Therapeutic Implications of Inflammation in the Pathogenesis of Brain Arteriovenous Malformations

Ashley Ricciardelli, Ariadna Robledo, Jason E. Fish. Peter T. Kan, Tajie H. Harris, Joshua D Wythe

Biomedicines, 2023

PMID: 38001877

 

Endothelial cells adopt a pro-reparative immune responsive signature during cardiac injury

Hali Long, Jeffrey D Steimle, Francisco Jose Grisanti Canozo, Jong Hwan Kim, Xiao Li, Yuka Morikawa, Minjun Park, Diwakar Turaga, Iki Adachi, Joshua D Wythe, Md Abul Hassan Samee, James F Martin.

Life Science Alliance, 2023

PMID: 38012001

 

Single-cell analysis identifies Ifi27l2a as a novel gene regulator of microglial inflammation in the context of aging and stroke.

Gab Seok Kim, Elisabeth Harmon, Manuel Gutierrez, Jessica Stephenson, Anjali Chauhan, Anik Banerjee, Zachary Wise, Andrea Doan, Ting Wu, Juneyoung Lee, Joo Eun Jung, Louise McCullough, Joshua Wythe, Sean Marrelli

Research Square (Preprint), 2023

PMID: 36824976

 

2022

 

Computational insights on coronary artery function.

David Mayerich, Joshua D Wythe

Nature Cardiovascular Research, 2022

PMID: 37564924

 

Development and characterization of a human arteriovenous malformation (AVM)-on-a-chip model.

Kayla Soon, Mengyuan Li, Ruilin Wu, Williamson D Turner, Joshua D Wythe, Jason E Fish, Sara S Nunes

Biomaterials, 2022

PMID: 35999080

 

EZ Clear for simple, rapid, and robust mouse whole organ clearing.

Chih-Wei Hsu, Juan Cerda, Jason M Kirk, Williamson D Turner, Tara L Rasmussen, Mary E Dickinson, Carlos P Flores Suarez, Joshua D. Wythe

eLife, 2022

PMID: 36218247

 

Defining the Transcriptional and Epigenetic Basis of Organotypic Endothelial Diversity in the Developing and Adult Mouse

Manuel E Cantu Gutierrez, Matthew C Hill, Gabrielle Largoza, James F Martin, Joshua D Wythe

bioRxiv

 

RBFOX2 is required for establishing RNA regulatory networks essential for heart development

Verma SK, Deshmukh V, Thatcher K, Belanger KK, Rhyner AM, Meng S, Holcomb RJ, Bressan M, Martin JF, Cooke JP, Wythe JD, Widen SG, Lincoln J, Kuyumcu-Martinez MN.

Nucleic Acids Research, 2022

PMID: 3513768

 

FOXO1 represses Sprouty2 and Sprouty4 expression in endothelial cells to promote arterial specification and vascular remodeling in the mouse yolk sac

Nanbing Li-Villarreal, Rebecca Lee Yean Wong, Monica D Garcia, Ryan S Udan, Ross A Poche, Tara L Rasmussen, Alexander M Rhyner, Joshua D Wythe, Mary E Dickinson

Development, 2022

PMID: 35297995

 

Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease

David Rohde, Katrien Vandoorne, I Lee, Jana Grune, Shuang Zhang, Cameron S McAlpine, Maximilian J Schloss, Ribhu Nayar, Gabriel Courties, Vanessa Frodermann, Gregory Wojtkiewicz, Lisa Honold, Qi Chen, Stephen Schmidt, Yoshiko Iwamoto, Yuan Sun, Sebastian Cremer, Friedrich F Hoyer, Oriol Iborra-Egea, Christian Muñoz-Guijosa, Fei Ji, Bin Zhou, Ralf H Adams, Joshua D Wythe, Juan Hidalgo, Hideto Watanabe, Yookyung Jung, Anja M van der Laan, Jan J Piek, Youmna Kfoury, Pauline A Désogère, Claudio Vinegoni, Partha Dutta, Ruslan I Sadreyev, Peter Caravan, Antoni Bayes-Genis, Peter Libby, David T Scadden, Charles P Lin, Kamila Naxerova, Filip K Swirski, Matthias Nahrendorf.

Nature Cardiovascular Research, 2022

PMID: 35747128

2021

 
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Identification and characterization of conserved noncoding cis-regulatory elements that impact Mecp2 expression and neurological functions

Shao Y, Bajikar SS, Tirumala HP, Gutierrez MC, Wythe JD, Zoghbi HY.

Genes & Development, 2021

PMID: 33737384

 
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Identification of diverse tumor endothelial cell populations in malignant glioma

Carlson JC^, Cantu Gutierrez M^, Lozzi B, Huang-Hobbs E, Turner WD, Tepe B, Zhang Y, Herman AM, Rao G, Creighton CJ, Wythe JD*, *Deneen B.

^equal contribution, *co-corresponding.

Neuro-Oncology, 2021

PMID: 33367832.

2020

 
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Somatic Gain of KRAS Function in the Endothelium is Sufficient to Cause Vascular Malformations that Require MEK but not PI3K Signaling

Fish JE^, Flores-Suarez CP^, Boudreau E^, Herman AM, Gutierrez MC,..….Cui M, Chen Z, Berman De Ruiz K, Schexnayder TS, Ward CS, Radovanovic I, Wythe JD. ^equal contribution.

Circulation Research, 2020.

PMID: 32552404 PMID: 32552404

 
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S1PR1 Regulates the Quiescence of Lymphatic Vessels by Inhibiting Laminar Shear Stress-dependent VEGF-C Signaling

Geng X, Yanagida K, Akwii RG, Choi D, Chen L, Ho Y, Cha B, Mahamud MR, Berman de Ruiz K, Ichise H, Chen H, Wythe JD, Mikelis CM, Hla T, Srinivasan RS.

JCI Insight, 2020.

PMID: 32544090.

 

Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung

Vila Ellis L, Cain MP, Hutchison V, Flodby P, Crandall ED, Borok Z, Zhou B, Ostrin EJ, Wythe JD, Chen J.

Developmental Cell, 2020.

PMID: 32059772.

 
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Development of barium-based low viscosity contrast agents for micro CT vascular casting: Application to 3D visualization of the adult mouse cerebrovasculature

Hong SH, Herman AM, Stephenson JM, Wu T, Bahadur AN, Burns AR, Marrelli SP, Wythe JD.

J Neurosci Res. 2020

PMID: 31630455

 

2019

 
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FishNET: An automated relational database for zebrafish colony management

Cantu Gutierrez ACantu Gutierrez MRhyner AM, Ruiz OE, Eisenhoffer GT, Wythe JD

PLoS Biology, 2019.

PMID: 31220074

 
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A cellular atlas of Pitx2-dependent cardiac development

Hill MC, Kadow ZA, Li L, Tran TT, Wythe JD, Martin JF.

Development, 2019. PMID: 31201182.

 

2018

 
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Perturbations of BMP/TGF-B and VEGF/VEGFR signaling pathways in non-syndromic sporadic brain arteriovenous malformations (BAVM)

Wang K, Zhao S, Liu B, Zhang Q, Li Y, Liu J, Shen Y, Ding X, Lin J, Wu Y, Yan Z, Chen J, Li X, Song X, Niu Y, Liu J, Chen W, Ming Y, Du R, Chen C, Long B, Zhang Y, Tong X, Zhang S, Posey JE, Zhang B, Wu Z, Wythe JD, Liu P, Lupski JR, Yang X, Wu N.  Journal of Medical Genetics, 2018.

PMID: 30120215

 
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Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development

Xiao Y, Hill MC, Zhang M, Martin TJ, Morikawa Y, Moise AR, Wythe JD, Martin JF. Developmental Cell, 2018

PMID: 29689192

 
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A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse

Herman AMRhyner AM, Devine WP, Marrelli SP, Bruneau BG, Wythe JD

Biology Open, 2018.

PMID: 29437553

 
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Somatic Activating KRAS Mutations in Arteriovenous Malformations of the Brain

Nikolaev SI, Vetiska S, Bonilla X, Boudreau E, Jauhiainen S, Rezai Jahromi B, Khyzha N, DiStefano PV, Suutarinen S, Kiehl TR, Mendes Pereira V, Herman AM, Krings T, Andrade-Barazarte H, Tung T, Valiante T, Zadeh G, Tymianski M, Rauramaa T, Ylä-Herttuala S, Wythe JD, Antonarakis SE, Frösen J, Fish JE, Radovanovic I. 

New England Journal of Medicine. 2018; 378(3):250-261.

PMID: 29298116.

 

2017

 

Dynamic Regulation Of VEGF-Inducible Genes By An ERK/ERG/P300 Transcriptional Network

Fish JE^, Cantu Gutierrez M^, Dang LT, Khyzha N, Chen Z, Veitch S, Cheng HS, Khor M, Antounians L, Njock MS, Boudreau E, Herman AM, Rhyner AM, Ruiz OE, Eisenhoffer GT, Medina-Rivera A, Wilson MD, Wythe JD.

^equal contribution.

Development. 2017; 144(13):2428-2444.

PMID: 28536097

 

2016

 
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Yap and Taz play a crucial role in neural crest-derived craniofacial development

Wang J, Xiao Y, Hsu CW, Martinez-Traverso IM, Zhang M, Bai Y, Ishii M, Maxson RE, Olson EN, Dickinson ME, Wythe JD, Martin JF.

Development. 2016; 143(3):504-15.

PMID: 26718006

 

2015

 
 

Repression of arterial genes in hemogenic endothelium is sufficient for haematopoietic fate acquisition

Lizama CO, Hawkins JS, Schmitt CE, Bos FL, Zape JP, Cautivo KM, Borges Pinto H, Rhyner AM, Yu H, Donohoe ME, Wythe JD, Zovein A.

Nature Communications, 2015; 6:7739.

PMID: 26204127

The molecular regulation of arteriovenous specification and maintenance

Fish JE, Wythe JD.

Developmental Dynamics, 2015; 244(3):391-409.

PMID: 25641373

 

University of California, San Francisco / Gladstone Institutes

2014

 
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Early patterning and specification of cardiac progenitors in gastrulating mesoderm

Devine WP, Wythe JD, George M, Koshiba-Takeuchi K, Bruneau BG.

Elife, 2014.

PMID: 25296024

 

2013

 

ETS factors regulate Vegf-dependent arterial specification

Wythe JD, Dang LT, Devine WP, Boudreau E, Artap ST, He D, Schachterle W, Stainier DY, Oettgen P, Black BL, Bruneau BG, Fish JE. 

Developmental Cell, 2013; 26(1):45-58.

PMID: 23830865