Site Specific Recombinases (SSRs): Cre & Genetic Reporters

 

Several site-specific recombinases (SSRs) exist, such as FlpE, Flpo, Phi31c, Cre and now Dre (among others). My principal interest in these arises from the ability to ablate a gene of interest's function in a temporally and spatially restricted manner (or "conditionally knockout"). For this type of technology, the SSR Cre, and its target sequence loxP, has been the default method of choice for several biologists. 

A second reason I am interested in SSRs is because I am very curious about cellular differentiation and the contribution of early progenitor cells to the developing embryo and adult. Accordingly, being able to visually identify early progenitors (or distinct populations) of cells and their descendants in a heritable, permanent fashion is highly desirable. This type of experiment is referred to as a genetic lineage tracing. For an excellent review on various lineage tracing strategies, see this comprehensive review from Legue and Joyner, 2010 and this paper from Buckingham and Meihlac, 2011 on clonal analysis.

Several SSR-dependent genetic reporter lines exist, but the lion's share are targeted to the Rosa26 locus and involve a loxP flanked "stop" cassette, or lox-stop-lox (or LSL) element blocking transcription from either the endogenous promoter at the Rosa locus or an artificial CAGGs promoter targeted to the Rosa locus. Thus, when Cre (or another SSR) acts on the sites to recombine out the intervening "stop" cassette, transcription of the reporter (in the image to the right, lacZ) is driven in all cells that express enough Cre molecules to induce recombination.

Briefly, for those not familiar, X-gal (5-bromo-4-chloro-3-indoyl-B-d-galactopyranoside) is a colorless, soluble chemical that has a galactose linked to an indole. B-galactosidase (encoded by lacZ) has a high Ka for the galactose part of the substrate and it hydrolyzes X-gal to release the indol, which dimerizes to produce the characteristic blue, insoluble product. Thus, in the presence of X-gal, all cell's that ever expressed enough Cre to recombine the sequence between the loxP sites at the Rosa locus, and all of their genetic descendants, will be labeled blue.

But what about other lineage reporter strategies? 

 

Cre diagram.png
 
 from http://www.tcichemicals.com/eshop/en/us/category_index/00317/

from http://www.tcichemicals.com/eshop/en/us/category_index/00317/